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The fitness of Mycobacterium smegmatis was affected negatively by oxaloacetate and positively by α-ketoglutarate, substrates of aspartate aminotransferase, which then altered the growth inhibition by antibiotics. Growth conditions ......................................................................................................................... Aspartate aminotransferases catalyze the reversible reaction of transamination between a dicarboxylic α-amino acid and the corresponding α-keto-acids by a ping-pong bi-bi mechanism with pyridoxal 5’-phosphate (PLP) as a cofactor [89]. tuberculosis genome contains a large conserved gene cluster involved in cholesterol catabolism as a carbon source during infection [90]. tuberculosis on lipids such as cholesterol is controlled by two Tet R- type regulators, Kst R (Rv3574) and Kst R2 (Rv3557c) [91,92].

Recombinant Asp B and Asp C both catalyze measurable transamination of aspartate and α-ketoglutarate. asp B belongs to a regulon controlled by the transcriptional repressor Kst R2 [92]. smegmatis de-repressed genes in the regulon including asp B, resulting in increased expression [92]. tuberculosis genome within an operon that includes fatty acid Co A ligase and acyl-Co A dehydrogenases [92].

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