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"The actual sample itself is collected while he was sedated for his safety and for the team's safety," Dr De Ploister said."Once he's under the anaesthetic it's a matter of massaging, and collecting a sample."He collected five millilitres of the leopard shark's semen into a syringe before looking at the sample under a microscope.

"We want lots of sperm to be present to increase our chances of getting a fertilised egg," he said.

All sharks have multiple rows of teeth, and while they lose teeth on a regular basis, new teeth continue to grow in and replace those they lose.

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Sharkbanz utilize powerful permanent magnets to create an effective shark deterrent that’s always on and requires no batteries or charging."We're looking at motility rate, how much sperm is swimming within the sample."We also want nice healthy sperm as well."Less than 24 hours after the extraction Dr De Ploister flew from Melbourne with the sample checked in as part of his luggage."The fresher the sample, the better the sample.I flew up in the afternoon and we did the artificial insemination on 10-year-old Honey [in Queensland] first thing in the morning," he said.Aaron Sprowl, general curator at SEA LIFE Mooloolaba, said the experiment had been carried out on the more common leopard shark, which is similar in many ways to the grey nurse."The advantage with a leopard shark is that it's a good size shark — big and long.They can get up to three metres long," Mr Sprowl said."They also behave similar to the grey nurse shark in that you can flip them over, hold their nose and do whatever procedures you need to do and then flip them back right side up and they go swimming off." With an estimated population of just 1,500 grey nurse sharks in the wild, and none in captivity, the marine scientists did not want to interfere with grey nurse reproduction.

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